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There is a growing need in molecular biology to interrogate samples for higher number of biomarkers, beyond the 2 to 5 biomarkers typically addressable with standard PCR technologies. Here, we demonstrate a novel approach to increase the level of multiplexing in digital PCR up to 15-plex by detecting each target with 2 distinct fluorophores with a 6-color digital PCR system, a method called digital PCR by color combination. We provide a statistical framework to interpret digital PCR data by color combination, predicting that high-plexed assays by color combination can, in theory, have the same precision and sensitivity as corresponding single-plex assays. We verified the statistical framework and confirmed the sensitivity prediction experimentally on a 15-plex assay and an 11-plex assay. These results establish the technical framework for future high-plex assays addressing a variety of applications and utilities, such as infectious disease syndromic panels or tumor mutation profiling panels.
https://www.biorxiv.org/content/10.1101/2023.05.10.540190v1
Stilla Technologies is a Paris-based European Biotechnology company that focuses on accelerating the development of next-generation genetic tests by providing a ground-breaking and flexible digital PCR (dPCR) solution: the naica® system. Taking advantage of cutting-edge microfluidic innovations, Stilla® aims to make dPCR a lab commodity in all Life Sciences areas: research, therapeutics, and all the “omics”. At Stilla Technologies, we continuously innovate and evolve technologically toward better human health outcomes. Our global team with multidisciplinary expertise shares a passion to develop new products for advanced genetic testing and analysis. Our expertise spans from microfluidics and chemistry to molecular biology and computer science. Our product, the naica® System, has been adopted by researchers & clinicians around the world to develop a wide range of genetic tests and molecular biology assays – including, but not limited to, liquid biopsy tests for cancer diagnostics, infectious diseases, prenatal testing, and GMO detection.
https://www.stillatechnologies.com/
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