Using EDTA as a Strategy to Reduce Protein Aggregation by Peak Proteins

Using EDTA as a Strategy to Reduce Protein Aggregation by Peak Proteins

Published on 27/09/2021

Using EDTA as a Strategy to Reduce Protein Aggregation by Peak Proteins

A client requested the large-scale production of a protein that had previously been optimised for purification during feasibility studies. This protein had proven tricky to purify during the feasibility stage and two attempts had been taken to establish a suitable purification strategy. High protein concentrations were found to be compromising the stability of the protein, so sample volumes were adjusted to minimise the risk. The buffer and pH conditions had also been optimised and the large-scale purification attempt was to be scaled up accordingly, to maximise soluble protein yield....read more here.

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